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Analytical Chemists Prof. Giuseppe Spoto & colleagues, Dipartimento di Scienze Chimiche, Università di Catania, Italy
Dr. Spoto is one of the most prolific users of GWC’s products. In collaboration with colleagues at several institutions, he uses GWC’s products for development of biosensors and materials science
applications. In one study, Dr. Spoto et al. used GWC’s SPRimager® platform to show that a synthetic Superoxide dismutase mimic interacts strongly with the lectin Ricinus communis agglutinin (RCA120). This model system is used to explore therapeutic approaches to inflammatory disease. Using kinetic analysis, this work yielded specific affinity measurements for the interaction.

Dr. Spoto’s group was also part of a collaboration to develop methods to monitor the activity of matrix metalloproteinases (MMPs), proteins involved in degrading the extracellular matrix. They anchored functional MMP catalytic domain to an SPRchip™, then determined surface coverage using GWC’s wavelength-scanning FT-SPR system. This analysis was followed by analysis using electrospray-ionization mass spectrometry to monitor MMP activity. The results establish a robust surface-based approach to monitoring MMP activity.

On the materials science front, Dr. Spoto was the first researcher to publish a paper that reports data obtained using all three GWC Technologies product platforms: SPR imaging, wavelength-scanning SPR and, using GWC’s SSD, PMIRRAS (reflectivity) measurements. This project showed that the electrochemically switchable Cu2+ complex of a 1,3 alternate bis(dipyridyl)calix[4]arene derivative forms self-assembled monolayers on Au(111) surfaces. The work is a key finding in the quest to develop novel microelectronic devices.
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